Abstract
The mitogen-activated protein kinase (MAPK) cascade is the most important mechanism in environmental responses and developmental processes in plants. The OsMAPK2 gene has been found to function in plant tolerance to diverse biotic/abiotic stresses. This paper presents evidence that OsMAPK2 (Oryza sativa MAP kinase gene 2) is responsive to Pi deficiency and involved in Pi homeostasis. We found that full-length expression of OsMAPK2 was up-regulated in both rice plants and cell culture in the absence of inorganic phosphate (Pi). The transgenic rice and Arabidopsis plants overexpressing OsMAPK2 showed affected root development and increased plant Pi content compared with wild-type plants. Overexpression of OsMAPK2 controlled the expression of several Pi starvation-responsive genes. Our results indicated that OsMAPK2 enables tolerance phosphate deficiency and is involved in Pi homeostasis.
Highlights
Rice is a monocot model plant, as well as an important cereal crop that supplies food for more than half of the world population
We examined the expression of OsIPS1 {a member of the Mt4/TPSI1 family in rice that is induced strongly by phosphate starvation [30]}, OsSPX1 {a negative factor for the accumulation of Pi in leaves [31]}, OsPHO2 {the potential ortholog of AtPHO2; its mutant is identified as a Pi overaccumulator [32]}, and the phosphate transporters (OsPT2, OsPT4 and OsPT8)
Our results suggest that OsMAPK2 plays a role in regulating the accumulation of Pi in Arabidopsis plants
Summary
Rice is a monocot model plant, as well as an important cereal crop that supplies food for more than half of the world population. Phosphate (Pi) is one of the most important nutrients for plant growth and development, but the availability of phosphate is frequently a limiting factor for crop productivity. This is because plants preferentially take up Pi as orthophosphate, and more than 80% of soil Pi is immobilized and is not readily available to roots [1,2]. In response to persistent Pi deficiency, plants have developed many adaptive mechanisms to enhance availability and increase the uptake of Pi, and plants exhibit morphological modifications and biochemical changes as well as regulate the expression of genes in response to cues in the phosphate signaling pathway [2,3]. It is important to study the functions of phosphate deficient condition responsive genes to increase the productivity and distribution of crop plants
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