Abstract
Overexpression of Act1 (CIKS) drives the expression of the human beta defensin gene, DEFB4, in a human bronchial epithelial cell line (HBE1). Act1 is an adaptor protein that is known to interact with I‐kappa‐kinases, as well as the interleukin‐17 receptor chain IL17RA, and is a potent activator of NFκB. We have previously shown that induction of DEFB4 by interleukin‐17 is largely NFκB dependent. Using DEFB4 promoter‐reporter constructs, we surprisingly show that induction of DEFB4 by Act1 overexpression is NFκB independent. Furthermore, we show that induction depends primarily on a region of the DEFB4 promoter that is located between −2.2 kb and −1 kb upstream of the transcriptional start site.This research was supported in part by grants from the NIH (HL077902, HL077315, ES00628)
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