Abstract
The Ca2+-dependent protein kinases (CDPKs) play important roles in Ca2+ signal transduction pathway in plant biotic stress response. In this study, AcCDPK1 and AcCDPK5 were isolated from onion. AcCDPK1 and AcCDPK5 showed higher expression in Phytophthora nicotianae resistant onion genotype compared to the susceptible onion line. AcCDPK1 and AcCDPK5 were cell membrane-localized proteins and contained conserved regions of plant CDPK protein, such as a serine‑ threonine protein kinase domain and a C-terminal calmodulin-like domain. AcCDPK1 and AcCDPK5 ectopic overexpression in Nicotiana benthamiana presented milder disease symptoms than that of wild type after P. nicotianae inoculation. The overexpression of AcCDPK1 and AcCDPK5 promoted the accumulation of ROS and upregulated the NbRBOHD transcript level at the initial stage of inoculation. In addition, AcCDPK1 and AcCDPK5 overexpression promoted the expression of NbPR1, which was related to the pathogen resistance. AcCDPK1 performed more obvious roles than AcCDPK5 in P. nicotianae resistance. The results suggested that AcCDPK1 and AcCDPK5 played a positive role in P. nicotianae resistance by regulating ROS accumulation and downstream resistance related genes expression.
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