Abstract
Members of the sucrose non-fermenting-1-related protein kinase 2 (SnRK2) family play central roles in the abscisic acid (ABA) signaling pathway and in mediating osmotic stress signaling and tolerance in plants. Previously, 12 full-length coding sequences that belong to SnRK2 gene family were identified and cloned in wild apple species Malus prunifolia. In this study, one of the members, MpSnRK2.10, was overexpressed in Arabidopsis and apple to investigate its potential function in response to drought stress. The results showed that overexpression of this gene did not affect plant growth under normal conditions. However, the transgenic plants showed enhanced tolerance to drought, as indicated by the amelioration in phenotype appearance and physiological indices related to drought stress damage. Additionally, transgenic apple plants overexpressing MpSnRK2.10 exhibited greater sensitivity to ABA compared to wild-type (WT) plants. Moreover, expressions of three stress response genes, MdRAB18, MdRD22, and MdRD29B, were more strongly induced in transgenic apple plants than in the WT when subjected to ABA and mannitol treatments. Taken as a whole, our study suggests that MpSnRK2.10 has a role in the enhancement of ABA signal transduction in response to stress, and that the manipulation of MpSnRK2.10 expression could be a feasible approach for improving abiotic stress tolerance in apple and other important crops.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
