Overexpressing the PRSS1 Protein Specifically in the Pancreatic Islets by Nano-Celery Provided Protection against Streptozotocin-Induced Diabetes Mellitus in Male Rat

Abstract

The goal of this study was to find out how giving whole barley grain to rats that had been given streptozotocin (STZ) affected lipid peroxidation (LPO), the activities of antioxidant enzymes in erythrocytes, and the production of insulin genes in the pancreas. A single shot of STZ (60 mg/kg, i.p.) caused diabetes in the experiment. The oxidative stress was measured by the amount of LPO in the tissue, the amount of reduced glutathione (GSH), and the activities of SOD, CAT, GPx, and GR enzymes in erythrocytes. The most important things that were seen in diabetic control rats were higher blood sugar and LPO levels, lower GSH levels, and lower enzyme functions. When diabetic rats (group G2) were given barley seed whole grain by mouth for 30 days, their LPO level dropped significantly compared to rats that were given STZ (group G3). Furthermore, diabetic rats (group G4) that were fed barley had higher levels of both enzyme- and non-enzymatic antioxidants, as well as higher levels of insulin and regeneration (PRSS1) genes, compared to diabetic normal rats (group G2). The results make it clear that oxidative stress plays a part in causing diabetes and suggest that adding barley to an animal's diet might help protect them.