Abstract
BackgroundCotton somatic embryogenesis is difficult or rarely frequent to present, which has limited gene function identification and biotechnological utility. Here, we employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop transgenic cotton callus for evaluating its function in ectopic plants.ResultsOverexpressing OsLOL1 can promote cotton callus to form embryogenic callus, not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells. And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector. Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene (GhSOD1) expression, possibly resulting in reactive oxidant species (ROS) accumulation in transgenic callus cells. And OsLOL1-overexpressed embryogenic callus exhibited higher α-amylase activity compared with the control, resulting from the promotion of OsLOL1 to cotton amylase 7 gene (GhAmy7) and GhAmy8 expression.ConclusionThe data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity, facilitating gene function analysis and molecular breeding in cotton.
Highlights
Plant somatic embryogenesis is a key process of cell totipotency and is an important type of plant regeneration as well as organogenesis plant regeneration
The results showed that overexpressed-OsLOL1 callus cells possessed higher capacity in transition of somatic-to- embryogenic callus cells and plant regeneration compared with the control callus cells transformed with empty vector
To increase cotton somatic embryogenesis and plant regeneration, we introduced OsLOL1 gene into the cotton chromosomes through Agrobacteriummediated transformation method
Summary
Plant somatic embryogenesis is a key process of cell totipotency and is an important type of plant regeneration as well as organogenesis plant regeneration. The increasing researches showed that some key genes regulate plant somatic embryogenesis and plant regeneration (Borderies et al 2004; Geshi et al 2013; Ledwoń and Gaj 2011; Long et al 2018; Nowak et al 2015; PérezPérez et al 2019; Poon et al 2012; Solís et al 2016; Stone et al 2008; Uddenberg et al 2016; Wang et al 2005; Zheng et al 2013). We employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop transgenic cotton callus for evaluating its function in ectopic plants
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