Abstract
BackgroundEthylene is an important industrial compound for the production of a wide variety of plastics and chemicals. At present, ethylene production involves steam cracking of a fossil-based feedstock, representing the highest CO2-emitting process in the chemical industry. Biological ethylene production can be achieved via expression of a single protein, the ethylene-forming enzyme (EFE), found in some bacteria and fungi; it has the potential to provide a sustainable alternative to steam cracking, provided that significant increases in productivity can be achieved. A key barrier is determining factors that influence the availability of substrates for the EFE reaction in potential microbial hosts. In the presence of O2, EFE catalyzes ethylene formation from the substrates α-ketoglutarate (AKG) and arginine. The concentrations of AKG, a key TCA cycle intermediate, and arginine are tightly controlled by an intricate regulatory system that coordinates carbon and nitrogen metabolism. Therefore, reliably predicting which genetic changes will ultimately lead to increased AKG and arginine availability is challenging.ResultsWe systematically explored the effects of media composition (rich versus defined), gene copy number, and the addition of exogenous substrates and other metabolites on the formation of ethylene in Escherichia coli expressing EFE. Guided by these results, we tested a number of genetic modifications predicted to improve substrate supply and ethylene production, including knockout of competing pathways and overexpression of key enzymes. Several such modifications led to higher AKG levels and higher ethylene productivity, with the best performing strain more than doubling ethylene productivity (from 81 ± 3 to 188 ± 13 nmol/OD600/mL).ConclusionsBoth EFE activity and substrate supply can be limiting factors in ethylene production. Targeted modifications in central carbon metabolism, such as overexpression of isocitrate dehydrogenase, and deletion of glutamate synthase or the transcription regulator ArgR, can effectively enhance substrate supply and ethylene productivity. These results not only provide insight into the intricate regulatory network of the TCA cycle, but also guide future pathway and genome-scale engineering efforts to further boost ethylene productivity.
Highlights
Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals
The efe gene was initially cloned into a high-copy plasmid downstream of an inducible lac promoter and transformed into E. coli (MG1655) cells
Previous work has demonstrated that ethylene-forming enzyme (EFE) protein accumulated in inclusion bodies in cultures grown at temperatures greater than 30 °C [9], so all of our cultures in this work were grown at 30 °C
Summary
Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals. Ethylene serves as a plant hormone, modulating growth and development and as a defense response to biotic and abiotic stresses [3, 4] Due to these roles, a variety of plant-associated pathogens and symbionts have evolved the ability to produce ethylene. While the precise roles ethylene may have in plant disease progression and symbiosis are unclear, evidence suggests that plants infected with certain ethylene-producing bacterial and fungal pathovars such as Pseudomonas syringae and Penicillium digitatum are compromised [5,6,7] These microbes use ethylene-forming enzyme (EFE) to catalyze the formation of ethylene in a single step. While the details of the EFE catalyzed reaction are still being determined [12], ethylene production via a single-enzyme conversion of common metabolites provides a straightforward means to produce bioethylene in engineered hosts such as E. coli
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
