Abstract

In some men with nonobstructive azoospermia caused by germ cell maturation arrest, spermatogenesis can be reactivated in vitro, either by culturing segments of explanted seminiferous tubules or by coculturing isolated germ cells on monolayers of somatic cell lines. Further studies are needed to compare the efficacy of these two culture systems in terms of restoration of fertilizing ability of in vitro–formed gametes and to evaluate the safety of the clinical use of these cells in assisted reproduction treatment.

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