Abstract
BackgroundLow-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid–AlCl3 catalyzed conversion to hydroxymethylfurfural (HMF) and levulinic acid (LA). However, lignin inhibits low-temperature swelling of TFA-treated intact wood particles from hybrid poplar (Populus tremula × P. alba) and results in greatly reduced yields of glucose or catalytic conversion compared to lignin-free cellulose. Previous studies have established that wood particles from transgenic lines of hybrid poplar with high syringyl (S) lignin content give greater glucose yields following enzymatic digestion.ResultsLow-temperature (− 20 °C) treatment of S-lignin-rich poplar wood particles in TFA slightly increased yields of glucose from enzymatic digestions and HMF and LA from maleic acid–AlCl3 catalysis. Subsequent gelatinization at 55 °C resulted in over 80% digestion of cellulose in only 3 to 6 h with high-S-lignin wood, compared to 20–60% digestion in the wild-type poplar hybrid and transgenic lines high in guaiacyl lignin or 5-hydroxy-G lignin. Disassembly of lignin in woody particles by Ni/C catalytic systems improved yields of glucose by enzymatic digestion or catalytic conversion to HMF and LA. Although lignin was completely removed by Ni/C-catalyzed delignification (CDL) treatment, recalcitrance to enzymatic digestion of cellulose from the high-S lines was reduced compared to other lignin variants. However, cellulose still exhibited considerable recalcitrance to complete enzymatic digestion or catalytic conversion after complete delignification. Low-temperature swelling of the CDL-treated wood particles in TFA resulted in nearly complete enzymatic hydrolysis, regardless of original lignin composition.ConclusionsGenetic modification of lignin composition can enhance the portfolio of aromatic products obtained from lignocellulosic biomass while promoting disassembly into biofuel and bioproduct substrates. CDL enhances rates of enzymatic digestion and chemical conversion, but cellulose remains intrinsically recalcitrant. Cold TFA is sufficient to overcome this recalcitrance after CDL treatment. Our results inform a ‘no carbon left behind’ strategy to convert total woody biomass into lignin, cellulose, and hemicellulose value streams for the future biorefinery.
Highlights
Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid–AlCl3 catalyzed conversion to hydroxymeth‐ ylfurfural (HMF) and levulinic acid (LA)
Because lignin interactions with cellulose are considered a major source of recalcitrance in biochemical conversion pathways, we evaluated the effect of modified lignin composition on the swelling and gelatinization of cellulose in woody biomass particles
We report here that catalytic delignification (CDL) using Ni/C doubles the rates of enzymatic hydrolysis of cellulosic residues of all poplar genetic variants, but higher initial rates of enzymatic digestion of the cellulosic residues were observed in the high-S-lignin lines
Summary
Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid–AlCl3 catalyzed conversion to hydroxymeth‐ ylfurfural (HMF) and levulinic acid (LA). Lignin inhibits low-temperature swelling of TFA-treated intact wood particles from hybrid poplar (Populus tremula × P. alba) and results in greatly reduced yields of glucose or catalytic conversion compared to lignin-free cellulose. AFEX swells lignocellulosic biomass, enabling nearly complete enzymatic conversion of cellulose to glucose [5]. Other treatments, such as NaOH/urea or 85% phosphoric acid, solubilize cellulose for improved saccharification yield [10]. Ionic liquids (ILs), such as 1-butyl-3-methylimidazolium (BMIM) chloride, or cellulose solvents, such as N-methylmorpholine N-oxide (NMMO), solubilize cellulose at relatively low temperatures without inducing extensive modification [11,12,13], by dissolving lignin and reducing cellulose crystallinity from woody biomass [14,15,16]. Production of ILs from lignin-rich residues promises to mitigate reagent costs [17], the recycling of such reagents together with recovery and separation of clean streams of desired reaction products remain energy-intensive processes in the context of biorefinery operations [18, 19]
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