Over-expression of miR-411–5p and miR-434–3p promotes the osteoblast differentiation by targeting GATA4

Abstract

ObjectiveTo investigate the role of miR-411–5p and miR-434–3p in osteoblast differentiation in particulate-induced osteolysis. MethodsA mouse model of osteolysis and an in vitro osteolysis model were constructed. The expressions of molecules were detected using qRT-PCR and western blot. Alkaline phosphatase (ALP) activity was measured using the ALP Assay Kit, and the bone mineralization was measured using alizarin red staining. ResultsThe expression of miR-411–5p and miR-434–3p was decreased in osteolysis mice and UHMWPE-induced mMSCs, while GATA4 protein expression was increased. Over-expression of miR-411–5p and miR-434–3p up-regulated the expressions of osteoblast gene markers, enhanced the ALP activity, promoted the bone mineralization of mesenchymal stem cells. In addition, miR-411–5p and miR-434–3p could target GATA4, and miR-411–5p/434–3p affected the expressions of osteoblast gene markers through GATA4 in vitro and in vivo. ConclusionOverexpression of miR-411–5p and miR-434–3p promoted the osteoblast differentiation by inhibiting GATA4 expression.