Over-Expression of HDA710 Delays Leaf Senescence in Rice (Oryza sativa L.).

Nannan Zhao,Zhen Su,Qian Song,Minghao Sheng,Chuanqing Sun,Xuelian Ma,Jie Zhao,Qiang Wei,Fengxia Liu,Wenying Xu,Kang Zhang

doi:10.3389/fbioe.2020.00471

Nannan Zhao, Zhen Su + Show 9 more

Open Access

https://doi.org/10.3389/fbioe.2020.00471

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Abstract

Histone deacetylases (HDACs) influence chromatin state and gene expression. Eighteen HDAC genes with important biological functions have been identified in rice. In this study, we surveyed the gene presence frequency of all 18 rice HDAC genes in 3,010 rice accessions. HDA710/OsHDAC2 showed insertion/deletion (InDel) polymorphisms in almost 98.8% japonica accessions but only 1% indica accessions. InDel polymorphism association analysis showed that accessions with partial deletions in HDA710 tended to display early leaf senescence. Further transgenic results confirmed that HDA710 delayed leaf senescence in rice. The over-expression of HDA710 delayed leaf senescence, and the knock-down of HDA710 accelerated leaf senescence. Transcriptome analysis showed that photosynthesis and chlorophyll biosynthesis related genes were up-regulated in HDA710 over-expression lines, while some programmed cell death and disease resistance related genes were down-regulated. Co-expression network analysis with gene expression view revealed that HDA710 was co-expressed with multiple genes, particularly OsGSTU12, which was significantly up-regulated in 35S::HDA710-sense lines. InDels in the promoter region of OsGSTU12 and in the gene region of HDA710 occurred coincidentally among more than 90% accessions, and we identified multiple W-box motifs at the InDel position of OsGSTU12. Over-expression of OsGSTU12 also delayed leaf senescence in rice. Taken together, our results suggest that both HDA710 and OsGSTU12 are involved in regulating the process of leaf senescence in rice.

Highlights

Epigenetic changes can reprogram the transcriptome during various biological processes
Rice Pan-genome Browser (RPAN) results showed that HDA710 was present in almost 98.8% JAP accessions, and 37.6% japonica-like ARO accessions, while it was only present in 1% of IND accessions and 0.9% of indicalike AUS accessions (Table 1)
We surveyed the gene presence frequency of all rice Histone deacetylases (HDACs) genes in 3,010 rice accessions, determining that deletions of fragments of HDA710 are significantly involved in the process of leaf senescence

Summary

Introduction

Epigenetic changes can reprogram the transcriptome during various biological processes. Acetylation and deacetylation of histones has emerged as a fundamental regulatory mechanism for the control of gene expression during plant development and in response to environmental conditions (Jang et al, 2003). Acetylation is a type of post-translational modification that changes as cells age, especially in animals (Sidler et al, 2017). With increasing age and senescence, H3K14ac, H4K8ac, and H4K12ac levels in mouse increase C. et al, 2007), and H3K56ac levels in mouse and human decrease (Dang et al, 2009; Feser et al, 2010; O’Sullivan et al, 2010), while H3K9ac and H4K16ac show different patterns in various species (Sidler et al, 2017)

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