Abstract
BackgroundThe aim of the present study was to analyze the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including patients' survival time.MethodsUsing real-time PCR, we detected the expression of EIF4G1 in normal nasopharyngeal tissues, immortalized nasopharyngeal epithelial cell lines NP69, NPC tissues and cell lines. EIF4G1 protein expression in NPC tissues was examined using immunohistochemistry. Survival analysis was performed using Kaplan-Meier method. The effect of EIF4G1 on cell invasion and tumorigenesis were investigated.ResultsThe expression levels of EIF4G1 mRNA were significantly greater in NPC tissues and cell lines than those in the normal nasopharyngeal tissues and NP69 cells (P < 0.001). Immunohistochemical analysis revealed that the expression of EIF4G1 protein was higher in NPC tissues than that in the nasopharyngeal tissues (P < 0.001). In addition, the levels of EIF4G1 protein in tumors were positively correlated with tumor T classification (P = 0.039), lymph node involvement (N classification, P = 0.008), and the clinical stages (P = 0.003) of NPC patients. Patients with higher EIF4G1 expression had shorter overall survival time (P = 0.019). Multivariate analysis showed that EIF4G1 expression was an independent prognostic indicator for the overall survival of NPC patients. Using shRNA to knock down the expression of EIF4G1 not only markedly inhibited cell cycle progression, proliferation, migration, invasion, and colony formation, but also dramatically suppressed in vivo xenograft tumor growth.ConclusionOur data suggest that EIF4G1 can serve as a biomarker for the prognosis of NPC patients.
Highlights
Nasopharyngeal carcinoma (NPC) is a common malignant disease in Southern China where its annual incidence rate is more than 20 cases per 100,000 populations
Latent membrane protein 1 (LMP1), a protein encoded by Epstein-Barr virus (EBV), is considered as a major oncogenic protein promoting the development of nasopharyngeal carcinoma (NPC)
Using realtime PCR to measure the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) transcripts, we found that EIF4G1 expression was significantly increased in freshly isolated NPC tissues (n = 10) and NPC cell lines (n = 6) in comparison to freshly isolated nasopharyngeal tissues (n = 7) and the immortalized human nasopharyngeal epithelial cell lines NP69 (Figure 1A) (P < 0.001)
Summary
Nasopharyngeal carcinoma (NPC) is a common malignant disease in Southern China where its annual incidence rate is more than 20 cases per 100,000 populations. Men are twice as likely to develop NPC as women. Because the primary anatomical site of tumor growth is located in a cryptic area and the disease is usually asymptomatic at early stages, the NPC patients tend to present at a more advanced stage with higher metastatic potential. Latent membrane protein 1 (LMP1), a protein encoded by Epstein-Barr virus (EBV), is considered as a major oncogenic protein promoting the development of NPC. The aim of the present study was to analyze the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including patients' survival time
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