Abstract
Honeybee populations infected by numerous viruses could not easily distinguish from the healthy populations because there are no apparent clinical symptoms and signs of infection. Black queen cell virus (BQCV) is one of the most prevalent viruses, resulting in the death of queen larvae and pupae. Like to other picornaviruses, BQCV has the RNA dependent RNA polymerase (RdRp) that catalyzes the replication of viral RNA. The optimal conditions for expression of MBP-BQCV-RdRp protein were confirmed by an induction with 0.1mM IPTG at 25°C for 6 hours and the MBP-BQCV-RdRp protein was purified using by amylose resin. Total yield of protein reached approximately 5mg per 50ml of induced cells. Due to its property that is an essential enzyme of virus replication, purified recombinant BQCV-RdRp proteins can be provided to the further lead as an important source to study how to control the BQCV viral disease in honeybee, development of a rapid kit for the diagnosis of BQCV infection in field,or finding out the anti-viral chemical compound for the inhibitor assay to prevent BQCV replication in honeybee.
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