Abstract
BackgroundIn mammals, the ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health. CircRNA is a non-coding RNA produced by non-canonical alternative splicing (AS). Several studies have reported that circRNA is involved in the gene regulation and plays an important role in some human diseases. However, the contribution of circRNA has received little known within the onset of puberty in ovary.ResultsHere, the profiles of ovarian circRNAs across pre-, in- and post-pubertal stages were established by RNA-sEq. In total, 972 circRNAs were identified, including 631 stage-specific circRNAs and 8 tissue-specific circRNAs. The biological functions of parental genes of circRNAs were enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras signaling pathway. Moreover, 5 circRNAs derived from 4 puberty-related genes (ESR1, JAK2, NF1 and ARNT) were found in this study. The A3SS events were the most alternative splicing, but IR events were likely to be arose in post-pubertal ovaries. Besides, the circRNA-miRNA-gene networks were explored for 10 differentially expressed circRNAs. Furthermore, the head-to-tail exon as well as the expressions of 10 circRNAs were validated by the divergent RT-qPCR and sanger sequencing.ConclusionsIn summary, the profiles of ovarian circRNAs were provided during pubertal transition in gilts, and these results provided useful information for the investigation on the onset of puberty at the ovarian-circRNAs-level in mammals.
Highlights
In mammals, the ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health
722 genes were identified as the parental genes of these 972 Circular RNA (circRNA). 922 exonic circRNAs were derived from 699 genes, and 23 intronic circRNAs were derived from 23 genes (Fig. 1 h)
In conclusion, we described the profiles of ovarian 972 circRNAs during pubertal transition in gilts, and these circRNAs were mostly enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras signaling pathway. 631 circRNAs were stage-specific, 8
Summary
The ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health. Several studies have reported that circRNA is involved in the gene regulation and plays an important role in some human diseases. HPO axis [10,11,12,13] the ovary is the female reproductive system and the endocrine organ, which produces the steroids and peptide hormones necessary for the onset of puberty [14,15,16]. Accumulating studies support that the noncoding RNA (ncRNAs) play a vital role in the expression of essential genes that regulate the oocyte growth and ovarian endocrine function [19, 20] as well as the onset of puberty [21, 22]
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