Abstract
This study aimed to analyze ovary histology and quantify total protein in the hemolymph of Rhipicephalus (Boophilus) microplus females treated with hexane extracts from green fruits of Melia azedarach. Eight engorged females were immersed in the extract at 0.25% concentration, and eight in water containing 5% acetone (control). The females were dissected 72 hours after treatment, and the ovaries were weighed and subjected to standard histological techniques. The total protein concentration was measured in the hemolymph of 200 females, of which 100 were treated as described above and 100 served as a control. In the treated group, ovary weight reduction and predominance of immature oocytes were observed. In addition, there were decreases in the diameters of the cytoplasm and germ vesicle of the oocytes in the treated group, compared with the controls. The protein concentration in the hemolymph was higher in the treated group than in the controls. The morphological changes observed in the treated ovaries included: presence of vacuolization; alteration of oocyte morphology, which changed from rounded to elongated; deformation of the chorion; and disorganization of the yolk granules. These results demonstrate the action of M. azedarach fruit extracts on R. (B.) microplus oogenesis.
Highlights
Rhipicephalus (Boophilus) microplus is a major parasitic problem for cattle producers, because of the economic losses caused by blood spoliation, predisposition towards myiasis, damage to leather, retardation of calf development, decreased meat production, excessive expenditure on control measures and transmission of pathogens that cause diseases such as anaplasmosis and babesiosis (JONSSON, 2006).The main method for controlling R. (B.) microplus is based on use of synthetic acaricides
These results demonstrate the action of M. azedarach fruit extracts on R. (B.) microplus oogenesis
The results from this study demonstrate the effect of crude hexane extracts from the fruits of M. azedarach on R. (B.) microplus oogenesis
Summary
Rhipicephalus (Boophilus) microplus is a major parasitic problem for cattle producers, because of the economic losses caused by blood spoliation, predisposition towards myiasis, damage to leather, retardation of calf development, decreased meat production, excessive expenditure on control measures and transmission of pathogens that cause diseases such as anaplasmosis and babesiosis (JONSSON, 2006).The main method for controlling R. (B.) microplus is based on use of synthetic acaricides. (B.) microplus is based on use of synthetic acaricides. The main motivation for searching for plant molecules as a possible tick control method arose from the perceived need to minimize the adverse effects of prolonged and intensive use of chemical acaricides (OLIVO et al, 2009). In this context, some plants such as Melia azedarach and Azadirachta indica (Meliaceae) stand out through contributing bioactive extracts for R. It is known that this plant has an effect on the neuroendocrine system of insects, which has been described by authors such as Schmidt et al (1998) and Mordue and Nisbet (2000). There is no information on its interference with the physiology of oogenesis in ticks
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