Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis

Abstract

Objective: To obtain the effective method of ovarian function preservation
 with granulose cell apoptosis assessment. Ovarian tissue
 vitrification became a method for ovarian function preservation in
 women with cancer. This technique can be done anytime without
 delay on cancer therapy both in prepubertal and unmarried patient.
 It can also store many primordial follicles. Ovarian tissue vitrification
 study is still limited to animal test and there are no data about apoptosis
 assessment after ovarian vitrification in human ovary.
 Method: This quasi experimental study was held in Department of
 Obstetrics and Gynecology Faculty of Medicine University of Indonesia
 - Dr. Cipto Mangunkusumo General Hospital and Fatmawati
 Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen
 women between 31 and 37 years old who underwent oophorectomy
 with gynecological indication were examined.
 Result: There was no morphological difference between follicles
 from fresh and warmed-vitrified ovaries. The mean protein Bax
 expression on the fresh ovaries assessed in the form of H-score
 was 1.66 (SD 0.14) compared with 1.68 (SD 0.13) on the warmedvitrified
 group (p=0.165). The mean protein Bcl-2 expression on the
 fresh ovaries examined in the form of H-score was 1.73 (SD 0.10)
 compared with 1.71 (SD 0.10) on the warmed-vitrified group
 (p=0.068).
 Conclusion: Ovarian tissue vitrification does not affect the Bax and
 Bcl-2 expression on human ovary.
 [Indones J Obstet Gynecol 2016; 4-2: 88-92]
 Keywords: apoptosis, bax, Bcl-2, ovarian tissue vitrification