Abstract
The increase of the world population generates the need to raise the production of food of vegetal and animal origin. In Brazil, livestock farming has evolved, demonstrating the important role of the country in the production of food. In this way, researchers of this productive chain have been looking for technologies related to production and reproduction, above all, to the use of reproduction biotechnologies, seeking to increase the production of different bovine breeds. Thus, the objective of this research was to investigate the efficacy of ovarian FSH super stimulation in bovine Wagyu oocyte donor females on follicular dynamics and in vitro embryo production. Twelve Wagyu animals, aged 12 to 24 months, randomly distributed in a crossover design were used in two groups: Group 1= animals not stimulated with FSH and Group 2= animals stimulated with FSH. The follicular, oocyte and embryonic variables were evaluated. It was observed that ovarian overstimulation in Wagyu oocyte donor cows with FSH improved the mean and large follicles but reduced the rate of oocyte recovery and, despite the best percentage of viable oocytes, there was no improvement in the amount of embryos produced in vitro.
Highlights
The United Nations Organization (UNO, 2017) estimates an increase in world population, reaching 8.6 billion people in 2030 and 9.8 billion in 2050
With the advent of artificial insemination (AI), producers were able to use bulls with high genetic value, and with the development of superovulation (SOV) and embryo transfer (ET) schemes, cattle breeding may increase the number of calves from females of high genetic standard (Hasler, 2014)
The animals were randomly assigned to a crossover design in two groups: Group 1 (G1): control - animals not stimulated with hormone = the experimental protocol of the cows of the Group 1 was to define a D0, a random day of the cycle, based on the commercial routine, on Day 8 was performed the follicular aspiration, lavage, oocyte classification and in vitro maturation (IVM), in Day 9 in vitro fertilization (IVF) and in Day 10, in vitro culture (IVC) (Figure 1)
Summary
The United Nations Organization (UNO, 2017) estimates an increase in world population, reaching 8.6 billion people in 2030 and 9.8 billion in 2050. In this way, researchers of several production chains, including beef, have studied technologies related to management, sanitation, nutrition and reproduction (Colombo et al, 2017; Cavalieri et al, 2019), seeking to increase the number of animals and / or the stocking rate of pastures, and increase the production of meat by area. With the advent of artificial insemination (AI), producers were able to use bulls with high genetic value, and with the development of superovulation (SOV) and embryo transfer (ET) schemes, cattle breeding may increase the number of calves from females of high genetic standard (Hasler, 2014). Studies evaluating several hormonal protocols have been developed to improve the reproductive indices of animals (Hasler, 2014)
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