Ovarian stimulation with follicle-stimulating hormone under increasing or minimal concentration of progesterone in dairy cows

Abstract

The objective of this study was to investigate the effect of the presence or absence of Corpus luteum (CL) on the follicular population during superstimulation in dairy cows (Holstein-Friesian cattle). Animals were divided into two groups as follows: (1) Growing CL group (G1): Cows (n = 7) received a total dose of 28 Armour units (AU) follicle-stimulating hormone (FSH) through the first 4 d (twice daily) after spontaneous ovulation (Day 0). (2) CL Absence group (G2): Cows (n = 10) received prostaglandin F 2α (PGF 2α) at 9 or 10 d after ovulation. After 36 h, all the follicles (larger than 5 mm) were aspirated (Day 0). The FSH treatment started 24 h after aspiration and continued for 4 d. The number of small (3 to <5 mm), medium (5 to <8 mm), and large (≥8 mm) follicles was examined on Days 1, 3, and 5 in all groups. Blood samples were collected daily for 5 d, and progesterone (P 4), estradiol (E 2), insulin-like growth factor-1 (IGF-1), and growth hormone (GH) in plasma were measured by enzyme immunoassays. The results showed that in G1, the P 4 level increased gradually from 0.5 ng/mL at Day 1 to 2 ng/mL at Day 5, whereas in G2, the P 4 level was completely below 0.5 ng/mL. All cows of the G2 group showed an increase of E 2 at Day 3 or Day 4 followed by an increase of IGF-1 within 24 h, while GH increased concomitantly with the E 2 increase in 8 of 10 trials. On the other hand, cows of the G1 group showed neither E 2 nor IGF-1 increase. Moreover, at the end of the treatment, the number of follicles in the G2 group was significantly increased compared with that of the G1 group (22.8 ± 2.0 vs. 11.6 ± 2.0). In conclusion, low P 4 level during FSH treatment enhanced multiple follicular growth and E 2 secretion, which was followed by increase of IGF-1 and GH. Therefore, the absence of the CL may play a critical role in the superovulation response by controlling the number of growing follicles.