Ovarian response, embryo production and hormonal profile in superovulated goats treated with insulin

Abstract

The influence of insulin on ovarian response and embryo production was investigated in 30 mixed breed goats, divided randomly into three equal ( n=10) groups. Goats in Group 1 (control) were superovulated using 20 IU FSH i.m. in six divided descending doses, i.e. 4/4, 3/3 and 3/3 IU at 12 h interval for three consecutive days and were not given insulin treatment. Goats in Group 2 (insulin pretreatment) were pretreated with long acting purified bovine insulin 0.2 IU/kg body weight per day s.c. on Days 7, 8 and 9 of the estrous cycle prior to initiation of superovulatory treatment as in Group 1. Animals in Group 3 (insulin cotreatment) were treated as in Group I, but in addition received long acting purified bovine insulin 0.2 IU/kg body weight per day s.c. as a cotreatment along with the first, third and fifth FSH treatments on three consecutive days. Total ovarian response (corpus luteum and unovulated large follicle (UOLF)) was significantly ( P<0.05) higher in insulin pretreatment (17.90±3.08) than in the cotreatment (11.50±2.34) and control (11.90±1.87) groups. The number of UOLF was significantly higher ( P<0.05) in the insulin pretreatment (10.2±1.67) than the cotreatment (4.9±1.14) and control (3.6±1.09) groups. The mean transferable quality of embryos did not differ significantly among treatments. Progesterone concentration on the day of PGF 2α treatment was not different ( P>0.05) between the insulin treatment groups (5.28±0.79; 5.30±0.66 ng/ml). Estradiol-17β concentration was significantly ( P<0.05) higher on the day of PGF 2α treatment in both the insulin treatment groups (36.67±6.40; 34.33±4.33 pg/ml) as compared to the control group (20.00±2.73 pg/ml). There is ample evidence to indicate beneficial effect of insulin on folliculogenesis and steroidogenesis in superovulated goats.