Ovarian follicular dynamics in heifers: Test of two-wave hypothesis by ultrasonically monitoring individual follicles

Abstract

The two-wave hypothesis for follicular development during the bovine estrous cycle was tested by ultrasonically monitoring individual follicles in 10 heifers during an interovulatory interval. A dominant follicle was defined as one that reached a diameter of at least 11 mm. Subordinate follicles were defined as those that appeared to originate from the same follicular pool as a dominant follicle. A dominant follicle and its cohorts were defined as a wave. Two waves during an interovulatory interval were identified in 9 of 10 heifers. The first wave was first identified, retrospectively, on a mean of Day 0.2 ± 0.1 (ovulation = Day 0) and gave origin to a dominant anovulatory follicle and a mean of 1.4 ± 0.3 identified subordinates. The dominant follicle reached maximum diameter (mean, 15.8 ± 0.8 mm) on an average of Day 7 and then decreased (P<.04) by Day 11. The subordinate follicles increased in diameter for a few days and then regressed. The second wave was first identified on a mean of Day 10.0 ± 0.4 and gave origin to the ovulatory follicle and a mean of 0.9 ± 0.3 subordinates. One of the 10 heifers had 3 waves of follicular activity characterized by an anovulatory wave emerging on Day 0, another anovulatory wave emerging on Day 10, and an ovulatory wave emerging on Day 16. Results strongly supported the two-wave hypothesis but also indicated that a minority of interovulatory intervals in this heifer population may have 3 waves of follicular activity.