Abstract
Background: Litter size is one of the most important economic traits that translates into higher returns for farmers. KISS 1 and NGF genes plays significant role in female reproduction. The purpose of the study was to evaluate the potential variation in the of expression levels of KISS1 and NGF genes in ovarian tissue of two different goat breeds with diverse prolificacy. Methods: Phenotypic records pertaining to litter size of Barbari and Jamunapari goats were collected. Total RNA was extracted from both breeds' ovarian tissues for real-time PCR quantification of the KISS1 and NGF genes, using GAPDH and β-actin as endogenous controls. The target gene’s expression levels were measured and the fold-change was determined. Result: The phenotypic recording revealed that Barbari goats are more prolific in nature than Jamunapari goats. The expression levels of KISS1 and NGF genes were higher in Barbari goat and lower in Jamunapari goat (P less than 0.05). The results of this study can be used to further discover the crucial role of KISS1 and NGF genes in reproduction for improving the prolificacy in goats.
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