Ovarian autoimmunity in relation to egg production in laying hens

Abstract

The aim of this study was to determine whether anti-ovarian autoantibodies appear in the circulation of laying hens and whether the concentrations of these antibodies change with respect to ageing and egg laying rate. Autoantibodies to ovarian tissues in the circulation of aged (aged approximately 670 days) White Leghorn hens with low (< 50%) and high (> 90%) egg laying rates were examined by ELISA and western blotting. Young laying hens (aged 185 days) with > 95% egg production were used as controls. The results of the ELISA indicated that IgG, which bound to the ovary and small white follicles, was present in the circulation of old laying hens. More hens that laid few eggs had circulatory autoantibodies to the ovary and small white follicles, as determined by the cut-off value in ELISA (mean absorbance + 2 SD of young laying hens), than did hens that laid greater numbers of eggs, and the concentration of IgG was significantly higher in the hens that laid few eggs. In contrast, when the muscle proteins were used as antigens there were no significant differences in the absorbance values among low and high laying frequency old hens or young hens. Western blotting revealed many bands of immunoprecipitates formed by ovarian antigens and antibodies in the serum of old hens, indicating the presence of many binding sites for circulatory IgG in ovarian tissues. These results indicate that antibodies to ovarian tissues appear in the circulation of laying hens during ageing, and that the concentration of these autoantibodies is related inversely to the rate of egg laying by hens.