Ovalbumin expression in the oviduct magnum of hens is related to the rate of egg laying and shows distinct stress-type-specific responses.

Abstract

Three trials were performed to evaluate the association of ovalbumin (OVA) abundance in the oviduct magnum with egg production and the underlying regulatory mechanism by glucocorticoids. In trial 1, twenty Hy-Line Brown layers (56-60weeks of age) with different combinations (n=5/combination) of laying rate (high or low) and egg weight (high or low) were selected from an initial group of 300. An upregulated expression of magnum OVA was observed (p<0.05) in hens with higher laying rate, regardless of egg weight. In trial 2, eighty Hy-Line Brown layers (80-90weeks of age) were subjected to the forced moulting (n=8). The abundance of OVA transcript and protein in the magnum was significantly decreased during moulting (p<0.01), and the same was true for laying rate (p<0.01) and serum oestrogen (p<0.05). In trial 3, forty-five 56-week-old Hy-Line Brown layers were kept individually (n=15) in the following conditions for 10days: constant optimal ambient temperature at 23°C and adlibitum feeding, high ambient temperature at 32°C for 6h/day (10:00-16:00) and adlibitum feeding (32AL), and constant optimal ambient temperature at 23°C and pair-fed to the 32AL hens. In spite of elevated corticosterone in circulation, OVA synthesis, blood oestrogen and laying rate were not affected by heat exposure (p>0.05). These results allow concluding that OVA expression in the oviduct magnum of hens is related to the rate of egg laying and shows distinct stress-type-specific responses.