Abstract
We review and propose several methods for identifying possible outliers and evaluate their properties. The methods are applied to a genomic prediction program in hybrid rye. Many plant breeders use ANOVA-based software for routine analysis of field trials. These programs may offer specific in-built options for residual analysis that are lacking in current REML software. With the advance of molecular technologies, there is a need to switch to REML-based approaches, but without losing the good features of outlier detection methods that have proven useful in the past. Our aims were to compare the variance component estimates between ANOVA and REML approaches, to scrutinize the outlier detection method of the ANOVA-based package PlabStat and to propose and evaluate alternative procedures for outlier detection. We compared the outputs produced using ANOVA and REML approaches of four published datasets of generalized lattice designs. Five outlier detection methods are explained step by step. Their performance was evaluated by measuring the true positive rate and the false positive rate in a dataset with artificial outliers simulated in several scenarios. An implementation of genomic prediction using an empirical rye multi-environment trial was used to assess the outlier detection methods with respect to the predictive abilities of a mixed model for each method. We provide a detailed explanation of how the PlabStat outlier detection methodology can be translated to REML-based software together with the evaluation of alternative methods to identify outliers. The method combining the Bonferroni-Holm test to judge each residual and the residual standardization strategy of PlabStat exhibited good ability to detect outliers in small and large datasets and under a genomic prediction application. We recommend the use of outlier detection methods as a decision support in the routine data analyses of plant breeding experiments.
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