Abstract
The increase of Zika virus (ZIKV) infections in Brazil in the last two years leaves a prophylactic measures on alert for this new and emerging pathogen. Concerning of our positive experience, we developed a new prototype using Neisseria meningitidis outer membrane vesicles (OMV) on ZIKV cell growth in a fusion of OMV in the envelope of virus particles. The fusion of nanoparticles resulting from outer membrane vesicles of N. meningitidis with infected C6/36 cells line were analyzed by Nano tracking analysis (NTA), zeta potential, differential light scattering (DLS), scan and scanning transmission eletronic microscopy (SEM and STEM) and high resolution mass spectometry (HRMS) for nanostructure characterization. Also, the vaccination effects were viewed by immune response in mice protocols immunization (ELISA and inflammatory chemokines) confirmed by Zika virus soroneutralization test. The results of immunizations in mice showed that antibody production had a titer greater than 1:160 as compared to unvaccinated mice. The immune response of the adjuvant and non-adjuvant formulation activated the cellular immune response TH1 and TH2. In addition, the serum neutralization was able to prevent infection of virus particles in the glial tumor cell model (M059J). This research shows efficient strategies without recombinant technology or DNA vaccines.
Highlights
The burden of ZIKV virus infection in Brazil and Latin America induced a run to produce new strategies to combat and prevent the infection and dissemination of this important emergent virus[1,2]
Soluble proteins are associated with Outer membrane vesicles (OMV) in the periplasm and externally as adhesive material
In order to characterize the quality of the ZIKV-OMV vesicles, all nanovesicles generated as described in Fig. 1, were analyzed in a Zetasizer equipment
Summary
The burden of ZIKV virus infection in Brazil and Latin America induced a run to produce new strategies to combat and prevent the infection and dissemination of this important emergent virus[1,2]. The severe clinical complications associated with ZIKV infection and the continuous circulation of this virus in invertebrate vectors and animals reservoirs (leading the possibility of new outbreaks in the near future), are illustrative of the urgent need to develop a vaccine against ZIKV14–17. In this scenario, the use of Outer membrane vesicles (OMV) vaccines could be a good strategy to develop an effective and cheap vaccine against ZIKV. Soluble proteins are associated with OMVs in the periplasm and externally as adhesive material Such nanovesicles may spread away from the cell and have different functions in the environment, mainly in a context with bacterial biofilm. OMVs act a delivery vesicles contributing for bacterial survival and virulence[29,30,31]
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