Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers

Carlos Antonio Fernandes Santos,Francisco Pinheiro Lima Neto

doi:10.1590/s0100-204x2011000800016

Abstract

The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (t m) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings.

Highlights

Mango (Mangifera indica L.) is one of the most important fruit crops in the Brazilian semiarid region, in which commercial orchards are based on Floridian varieties, and 'Tommy Atkins', is cultivated in 85% of an estimated area of 25,000 ha
A 'Haden' tree isolate was identified in a commercial orchard of 'Tommy Atkins' at a mango‐exporting farm, in Petrolina, PE, Brazil
Fruits were harvested from the 'Haden' tree, that was at a minimum distance of 200 m from cultivars other than Tommy Atkins, to avoid crosses different from 'Haden' x 'Tommy Atkins'

Summary

Introduction

Mango (Mangifera indica L.) is one of the most important fruit crops in the Brazilian semiarid region, in which commercial orchards are based on Floridian varieties, and 'Tommy Atkins', is cultivated in 85% of an estimated area of 25,000 ha. This situation could have a negative impact on the local mango agribusiness, considering changes in overseas consumer preferences or a severe infestation of a specific pest or disease, highlighting the importance of the development of new mango cultivars. The anthesis is diurnal, asynchronic, and releases a strong and sweet odor. According to Jison & Hedstron (1985), the mango flower structure is totally adapted to cross pollinization, and Apis mellifera, Belvosia bicincta, and Musca domestica are the most frequent insect visitors to 'Tommy Atkins' in the São Francisco valley (Siqueira et al, 2008)

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