Outcome of stimulation of endometrium embryo transfer (SEET): from an analysis of 2,168 single frozen-thawed blastocyst transfer cycles

Abstract

SEET is a variant method of blastocyst transfer, which is characterized by the injection of the embryo culture supernatant into the uterus before blastocyst transfer. We reported that SEET improved the implantation rate and the pregnancy rate in single frozen-thawed blastocyst transfer cycles (Fertil Steril 2007, 2009). Thereafter, we primarily select SEET method in frozen-thawed blastocyst transfer cycles. In this study, we retrospectively analyzed the outcome of SEET cycles in our ART center. Retrospective analysis. In this study, we collected a total of 2168 cycles of SEET cycles between January 2009 and December 2010. SEET method was performed as previously described (Fertil Steril 2007). We stratified data according to age, previous ART history, and morphological grade of blastocyst, and then investigated the clinical outcome in each group. The average age was 36.3, the average number of previous ART cycles was 2.4, and the total clinical pregnancy rate was 38.4%. The more previous ART cycles led to the lower clinical pregnancy rate (Table1). The clinical pregnancy rates of patients under 37 years of age and above 38 years of age were 45.1% and 27.8%, respectively. The clinical pregnancy rates after high grade blastocyst transfer and low grade blastocyst transfer were 57.0% and 31.9%, respectively.Tabled 1Number of previous ART cycles and Clinical outcome.No. of previous ART12345≤No. of transfer cycles141040016663129Age of patient (y)35.5 ± 4.036.2 ± 3.937.5 ± 3.639.9 ± 3.741.0 ± 3.8No. of chemical pregnancies9342451022650Implantation rate per embryo (%)66.261.361.441.338.8No. of clinical pregnancies582150591526Clinical pregnancy rate per transfer (%)41.337.535.523.820.2No. of pregnancy loss102361836pregnancy loss rate (%)17.52430.52023.1 Open table in a new tab In this study, we showed the good clinical outcome of SEET. SEET is a simple and effective method for single blastocyst transfer.