OUTCOME OF IN-VITRO FERTILIZATION AND THE AUTO ANTIBODIES AGAINST ANNEXIN V AND CARDIOLIPIN

Abstract

Objective: To evaluate the role of anti-annexin V antibodies (aANX) and anticardiolipin antibodies (ACA) as riskfactors and predictors for in vitro fertilization-embryo transfer (IVF or ICSI) outcome.Design: Prospective study,Setting: IVF unit, Int. Islamic center for population studies and researches, Al-Azhar University, and Health centers in10lh of Ramadan city.Subjects and Methods: Were classified into 3 groups:-Group I: 30 women had one or more failed IVFJET despite good morphological quality of the transferred embryos.Group II: 30 non-pregnant women had previous history of live births.Group III: 30 pregnant women in the first trimester as controls previously delivered normally and no previous historyof pregnancy loss. All were submitted to:1) Detailed history to eliminate any case with renal, hepatic, or metabolic diseases.2) Complete medical examination and laboratory investigations to exclude autoimmune disease, infection. hypertension, D.M., or other endocrinal disease.3) a- Serum antiannexin V antibodies were measured by Eliza. b-Serum anticardiolipin antibodies were measured by Eliza.Results:1) Serum antiannexin V IgG and serum anticardiolipin IgG were increased significantly in group I compared to the other groups.2) There was no significant difference between group II&III for both parameters of antiannexin V IgG & anticardiolipin IgG with lower level in group III than group II.Conclusion: The antiannexin V IgG & anticardiolipin IgG both can be used as predictors for IVF-ET success.