Abstract
Clupeiformes, such as sardines and herrings, represent an important share of worldwide fisheries. Among those, the European sardine (Sardina pilchardus, Walbaum 1792) exhibits significant commercial relevance. While the last decade showed a steady and sharp decline in capture levels, recent advances in culture husbandry represent promising research avenues. Yet, the complete absence of genomic resources from sardine imposes a severe bottleneck to understand its physiological and ecological requirements. We generated 69 Gbp of paired-end reads using Illumina HiSeq X Ten and assembled a draft genome assembly with an N50 scaffold length of 25,579 bp and BUSCO completeness of 82.1% (Actinopterygii). The estimated size of the genome ranges between 655 and 850 Mb. Additionally, we generated a relatively high-level liver transcriptome. To deliver a proof of principle of the value of this dataset, we established the presence and function of enzymes (Elovl2, Elovl5, and Fads2) that have pivotal roles in the biosynthesis of long chain polyunsaturated fatty acids, essential nutrients particularly abundant in oily fish such as sardines. Our study provides the first omics dataset from a valuable economic marine teleost species, the European sardine, representing an essential resource for their effective conservation, management, and sustainable exploitation.
Highlights
Teleosts comprise the most species-rich group of vertebrates, with approximately 30,000 describedTeleosts comprise the decades, most species-rich group of as vertebrates, with approximately described species [1]
To demonstrate the biological value of the omics datasets, we investigated the key enzymes of LC-PUFA biosynthesis in the sardine draft genome and liver transcriptome, a major metabolic site for PUFA metabolism [15]
We examined the function of the enzymes encoded by the fads2, elovl2, and elovl5
Summary
Teleosts comprise the most species-rich group of vertebrates, with approximately 30,000 described. Numerous teleost fish species are of high commercial importance for evolutionary [2]. Teleost fish species are ofofhigh commercial importance for both fisheries studies and aquaculture. The oily fish[6], European sardine (Sardina pilchardus, Walbaum one of theconsumed most commercially important species for the canning industry, and has 1792). . Here, we de present assemblies based on low coverage and short read approaches are cost effective, and provide valuable the first draft genome of the European sardine and provide a relatively high-level liver transcriptome biological information studies [11,12,13].inHere, we present first draft genome sardine was and enabling nutrigenomics this iconic species.the.
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