Abstract
AbstractFrom different fungal genera some mutant strains were selected which in cellulase formation exhibit reduced catabolite‐repression related to several soluble carbon sources. The cellulase production by these mutant strains on the basis of soluble carbon sources is possible if a fed‐batch‐technique is used for feeding the substrate.The optimum procedure is an oscillating‐fed‐batch‐technique (OFB) with feed‐back‐controlled intermittent addition of the substrate. The substrate concentration in the medium is adjusted in such a manner to realize oscillations between a limitation for mycelium growth and non‐limiting conditions under a substrate concentration which is yet below the repressing concentration for cellulase formation. A suitable feed‐back‐parameter for the control of substrate addition is the specific CO2‐formation of the mycelium.Using OFB the growth in cellulase activity is nearly proportional to the feeded substrate whereas the growth in mycelium is reduced. The productivity of cellulase formation is enhanced in comparison to continuous substrate addition as well as under limiting and non‐limiting conditions for growth of mycelium.
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