Abstract
Purpose To study the effect of osthole extract on proliferation, migration, invasion, and apoptosis of human cervical carcinoma HeLa cells and investigate its underlying mechanism. Methods HeLa cells were exposed to osthole at various concentrations. Cell viability, migration, and invasion were detected by MTT assay, scratch wound-healing assay, and invasion assay, respectively. The proportion of cells undergoing apoptosis was analyzed by flow cytometry. Western blot and RT-qPCR were performed to determine changes in the expression of key factors in the Wnt/β-catenin signaling pathway. Results The osthole extract effectively inhibited the proliferation, migration, and invasion potential of HeLa cells in a dose-dependent manner. The rate of apoptosis induction in HeLa cells treated with the osthole extract for 48 h was significantly higher than that of the untreated controls. Outcomes of the western blotting analysis and RT-qPCR showed that the expression of β-catenin, c-Myc, cyclin D1, survivin, and MMP-9 was significantly inhibited. Conclusion Osthole could significantly inhibit the malignant behavior of HeLa cells and induce cellular apoptosis. Inactivation of the Wnt/β-catenin signaling pathway by osthole may be a mechanism to control cancer metastasis.
Highlights
Cervical cancer is one of the most common female genital malignancies worldwide that pose a serious threat to the life and health of women aged 20 to 39 years [1, 2]
The human cervical carcinoma HeLa cell line was obtained from the molecular biology testing center of the Henan University of Chinese Medicine
We evaluated the antitumor effects of osthole on HeLa cells and investigated the potential mechanism
Summary
Cervical cancer is one of the most common female genital malignancies worldwide that pose a serious threat to the life and health of women aged 20 to 39 years [1, 2]. Statistics (2016) showed that about 520,000 new cases of cervical cancer were diagnosed annually, and nearly 70,000 people died from this fatal disease [3]. Combined radiotherapy and chemotherapy remains the mainstay of treatment for cervical cancer and has demonstrated significant progress in cancer control. Clinical complications of anticancer therapies or their severe side effects cannot be ignored. Chinese medicine therapy has shown remarkable potential as an alternative for cervical cancer treatment or as auxiliary reagents that support cancer treatment owing to their high efficiency and low toxicity [4]. In vitro and in vivo studies on human cervical cancer suggested that Guizhi-Fuling-decoction, a traditional
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