Osteoprotegerin expression and serum values in obese women with type 2 diabetes mellitus.

Nearmeen M Rashad,Sally M Shalaby,Walaa M Sarhan,Amal S El-Shal,Hanim M Abdel-Nour

doi:10.1007/s11033-021-06699-x

Nearmeen M Rashad, Sally M Shalaby + Show 3 more

Open Access

https://doi.org/10.1007/s11033-021-06699-x

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Abstract

BackgroundObesity and diabetes prevalence are increasing worldwide. We aimed to detect the possible association of osteoprotegerin (OPG) gene expression with visceral adiposity indices and cardiometabolic risk factors among obese women.Methods and resultsThe study enrolled 150 controls and 150 obese cases subdivided into two subgroups non-diabetic (n = 70) and 80 patients with type 2 diabetes mellitus (T2DM). Circulating OPG gene expression levels were figured out by real time PCR (Polymerase Chain Reaction). Serum OPG levels were assessed by Enzyme Linked Immunosorbent Assay. Our results explored that OPG serum levels were lower in the obese women compared to control group (p < 0.001) and obese diabetics had higher serum levels of OPG in comparison to obese non-diabetic patients (p < 0.001). Expression levels of OPG were higher in obese women than controls (p < 0.001). Moreover, the blood expression levels of OPG gene were higher in diabetic obese patients than non-diabetics. We found positive correlations between parameters of metabolic syndrome and obesity indices. After adjustment of the traditional risk factors, stepwise linear regression analysis test revealed that OPG expression levels were independently correlated with glycated hemoglobin, high-density lipoprotein-cholesterol, and waist-to-hip ratio.ConclusionsOPG mRNA levels were associated with surrogate markers of insulin resistance in Egyptian obese women.

Highlights

The obesity pandemic is considered a major public health problem, as in this disorder there is increased risk of medical morbidity leading to a significant rise in death rate
Obese women were categorized into two subgroups according to their fasting blood glucose (FBG) based criteria reported by the American Diabetes Association [ADA] in 2015 into: non-diabetic patients (FBG < 126 mg/ dl) (n = 70) and 80 cases with type 2 diabetes mellitus (T2DM) (FBG ≥ 126 mg/dl)
All obesity indices and parameters (WC, body mass index (BMI), WHR, WHtR, Body adiposity index (BAI) and Visceral adiposity index (VAI)) were significantly higher in obese women compared to leans

Summary

Introduction

The obesity pandemic is considered a major public health problem, as in this disorder there is increased risk of medical morbidity leading to a significant rise in death rate. A particular issue in Egypt is that prevalence of obesity is more than double among females (46%) as compared to males (22%) [3]. This is a growing concern as obese subjects are at increased risk of developing diabetes, high blood pressure, dyslipidemia, in addition to coronary heart disease, stroke and cancers [4]. We aimed to detect the possible association of osteoprotegerin (OPG) gene expression with visceral adiposity indices and cardiometabolic risk factors among obese women. Expression levels of OPG were higher in obese women than controls (p < 0.001). The blood expression levels of OPG gene were higher in diabetic obese patients than non-diabetics. Conclusions OPG mRNA levels were associated with surrogate markers of insulin resistance in Egyptian obese women

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