Abstract
BackgroundOsteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. This study was carried out to investigate the role of HOTAIR in OA.MethodsSynovial fluid was extracted from both OA patients (n = 58) and healthy controls (n = 58). Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 µg/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay.ResultsHOTAIR was upregulated in OA. A positive correlation between HOTAIR and osteopontin was observed. In the primary chondrocytes, osteopontin treatment increased HOTAIR expression, while HOTAIR overexpression and knockdown failed to significantly affect osteopontin expression. In addition, osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIRE knockdown decreased chondrocyte proliferation. In addition, HOTAIR knockdown reduced the effects of osteopontin treatment on cell proliferation.ConclusionsOsteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
Highlights
Osteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts
HOX transcript antisense RNA (HOTAIR) and osteopontin were upregulated in OA Synovial fluid samples isolated from both OA patients (n = 58) and healthy controls (n = 58) were subjected to RNA isolation and RT-qPCR to measure the mRNA expression levels of HOTAIR and osteopontin
Our data suggested that upregulated expression of HOTAIR and osteopontin may involve in OA
Summary
Osteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 μg/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay. Osteopontin, known as Secreted Phosphoprotein 1 (SPP1), is a multifunctional phosphoprotein that is secreted by multiple cells types, such as chondrocytes and osteoblasts, is upregulated in OA and induces the production of matrix
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