Abstract
The accumulation and formation of advanced glycation end products (AGEs) are related to diabetes and age-related disease. Osteomeles schwerinae C. K. Schneid. (Rosaceae, OSSC) is used traditionally for the treatment of various diseases in Asia. Previous studies have shown that OSSC elicits preventive effects in an in vivo model of diabetes. This study was to evaluate the antiapoptotic effects of dried leaves and twigs of OSSC extract and its major compounds in ARPE-19 cells—spontaneously arising human retinal pigment epithelial cells—under diabetic conditions. To examine the effects of an OSSC extract and its active compounds (acetylvitexin, hyperoside and quercitrin) on apoptosis in methylglyoxal (MG, the active precursor in the formation of AGEs)-treated ARPE-19 cells and the mechanism by which these effects occur, apoptosis was measured using flow cytometry analysis. Protein expression levels of phospho-p53 (p-p53), Bax and Bcl-2 were determined by western blot analyses. The OSSC extract inhibited apoptosis in MG-treated ARPE-19 cells in a dose-dependent manner. The major compounds also reduced the rate of apoptosis. Both the extract and major compounds also inhibited the expression of p-p53 and Bax and increased the levels of Bcl-2 that had been previously reduced by MG treatment. The OSSC extract (0.1 μg/mL) and its major compounds (0.01 μM) attenuated apoptosis in ARPE-19 cells under toxic diabetic conditions by downregulating of expression of p-p53 and Bax. OSSC may serve as an alternative therapy to retard the development of diabetic retinopathy.
Highlights
The accumulation of advanced glycation end products (AGEs) is accelerated under conditions of chronic hyperglycemia and many age-related diseases [1]
To assess the effects of the Osteomeles schwerinae C. K. Schneid. (OSSC) extract and its maker compounds on apoptosis-related factors involved in apoptosis, we examined the expression levels of p-p53, Bax and Bcl-2 by western blot analysis
The results showed that the OSSC extract and its maker compounds significantly inhibited apoptosis under diabetic conditions
Summary
The accumulation of advanced glycation end products (AGEs) is accelerated under conditions of chronic hyperglycemia and many age-related diseases [1]. Methylglyoxal (MG), a highly reactive dicarbonyl compound, is an AGE precursor whose levels have been shown to increase in experimental and clinical diabetes [1]. Elevated MG levels have been shown to induce reactive oxygen species (ROS) production and apoptosis in various. (OSSC), belonging to the family Rosaceae, is a perennial evergreen shrub, native to China that is harvested in the wild and used for local food [9]. This plant that has been used as a traditional Chinese medicine for the treatment of laryngopharyngitis, diarrhea, dysentery, folliculitis and hyperglycemia [10,11].
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