Osteogenic Property Regulation of Stem Cells by a Hydroxyapatite 3D-Hybrid Scaffold With Cancellous Bone Structure

He Xia,Xin Chen,Yuan Wei,Haijun Li,Yuanhua Sang,Min Hao,Hong Liu,Jiazhi Duan,Liyang Yu,Lun Dong

doi:10.3389/fchem.2021.798299

Abstract

Cancellous bone plays an indispensable role in the skeletal system due to its various functions and high porosity. In this work, chitosan and hydroxyapatite nanowires (CS@HAP NWs) hybrid nanostructured scaffolds with suitable mechanical properties, high porosity and a fine porous structure were prepared to simulate the 3-dimensional structure of cancellous bone. The 3D-hybrid scaffolds promote cell adhesion and the migration of human adipose-derived stem cells (hADSCs) inside the scaffolds. The cavities in the scaffolds provide space for the hADSCs proliferation and differentiation. Moreover, the various contents of HAP and the induced mechanical property changes regulate the differentiation of hADSCs toward osteoblasts. Overall, cellular fate regulation of hADSCs via rationally engineered HAP-based hybrid scaffolds is a facile and effective approach for bone tissue engineering.

Highlights

Bone defects caused by trauma, congenital anomalies and tumor resection create a huge demand for bone grafts (Zhang et al, 2019; Koons et al, 2020; Collins et al, 2021)
For the cell experimental process, the α−minimum essential medium (α−MEM), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and penicillin/ streptomycin were purchased from Gibco (America)
hydroxyapatite nanowires (HAP NWs) were synthesized by the hydrothermal method

Summary

Introduction

Bone defects caused by trauma, congenital anomalies and tumor resection create a huge demand for bone grafts (Zhang et al, 2019; Koons et al, 2020; Collins et al, 2021). With the progression of tissue engineering, biomimetic bone scaffolds can guide tissue regeneration with assembled stem cells (Zhao et al, 2019; Swanson et al, 2021; You et al, 2021). They have considered a cost-effective and osteoinductive strategy to replace autologous bone grafts (Cui et al, 2019). There are many studies concerning the modification of the matrix to provide a suitable bioenvironment for the osteogenic property regulation of stem cells (Jo et al, 2017; Yang et al, 2018; Wang et al, 2019; Zhong et al, 2021).

Methods

Results

Conclusion