Abstract
BackgroundThe aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone.MethodsThe experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(−)DC and Dex(−)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(−)DC, Dex(−)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR).ResultsIn this study, we assessed osteogenic differentiation of BMSCs on Duck’s feet-derived collagen (DC)/HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days.ConclusionFrom these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.
Highlights
The aim of this study was to investigate the osteogenesis effects of derived collagen (DC) and DC/HAp sponge immersed in without and with dexamethasone
We have evaluated osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) on duck’s feet-derived collagen (DC) sponges immerged in Dex (Dex(+)-DC)
BMSC was cultured on theses sponges in Dex(+), Dex(−) medium
Summary
The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone. An ideal scaffold for tissue engineering and regenerative medicine should have the following characteristics such as biocompatibility, good porosity, pore size, sufficient mechanical strength and good cell attachment [1]. The scaffold containing cells and bioactive substance components plays critical roles, which supports the transplanted cells and maintains bio-functions effectively [2,3,4,5,6,7,8,9]. The methods of fabricating scaffolds with cells and bioactive molecules have been developed. Collagen is generally used as a biomaterial for tissue engineering and has been known for diverse biomedical application in bone tissue engineering because of their excellent biocompatibility, low immunogenicity and biodegradability [13,14,15,16,17,18,19,20]. Engineered collagen scaffold have been appropriate for
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