Osteoblast differentiation from synovial fluid cells in juvenile idiopathic arthritis (JIA)

Marija Jelusic-Drazic,Elvira Lazic Mosler,Mandica Vidovic,Danka Grcevic,Natasa Kovacic,Ana Marusic

doi:10.1186/1546-0096-10-s1-a115

Abstract

Purpose Juvenile idiopathic arthritis (JIA) is one of the leading causes of disability in children characterized by destruction of articular cartilage and underlying bone, as well as synovial hyperplasia. Since synovia contains osteoblast progenitors, hyperplastic changes may contribute to joint destruction by inhibition of osteoblast differentiation. Osteoblasts are also immunoregulatory cells and their inhibited differentiation and function may affect joint inflammation. The objective of this report is to explore the osteoblastogenic potential of synovial fluid cells in JIA and to examine whether synovial fluid from children with JIA effects osteoblast differentiation of human bone marrow cells in vitro. Additionaly, we aimed to determine and compare the local and systemic expression of osteoblast related genes in patients with JIA.

Highlights

Juvenile idiopathic arthritis (JIA) is one of the leading causes of disability in children characterized by destruction of articular cartilage and underlying bone, as well as synovial hyperplasia
Blood samples were obtained from 21 children with oligoarticular JIA, 21 children with polyarticular JIA, and 24 control children
Gene expression of Runx1, Runx2, Runx3 and receptor activator of nuclear factor kappa-B ligand (RANKL) was higher in synovial fluid of children with polyarticular JIA (pJIA) than with oligoarticular JIA (oJIA).The expression of osteoblast related genes in peripheral blood mononuclear cells from children with JIA was similar to healthy controls

Summary

Introduction

Juvenile idiopathic arthritis (JIA) is one of the leading causes of disability in children characterized by destruction of articular cartilage and underlying bone, as well as synovial hyperplasia. Since synovia contains osteoblast progenitors, hyperplastic changes may contribute to joint destruction by inhibition of osteoblast differentiation. Osteoblasts are immunoregulatory cells and their inhibited differentiation and function may affect joint inflammation. The objective of this report is to explore the osteoblastogenic potential of synovial fluid cells in JIA and to examine whether synovial fluid from children with JIA effects osteoblast differentiation of human bone marrow cells in vitro.

Objectives

Methods

Results