Abstract
Plants germin-like proteins (GLPs) and auxin binding proteins are involved in response to various phytohormones including auxin, pathogen infestations and abiotic stresses. Rice GLPs are involved in sheathing against blast pathogens. Keeping in view the role of GLPs in plant defence, the present study was conducted to investigate the expression analysis of rice OsRGLP2 (Oryza sativa root germin-like protein 2) promoter in response to drought, salt stress, auxins, H2O2 and salicylic acid (SA) in transgenic tobacco. A decrease in GUS expression was observed with an increase in PEG-6000 concentration while OsRGLP2 expression was strongly induced in response to SA. The maximum promoter-derived GUS expression of OsRGLP2 was noted with 300 mM NaCl after 2 h of application and lower activity in the following hours. OsRGLP2 promoter activity was observed in response to all given concentrations of H2O2 with the maximum expression at 1 and 5 mM of H2O2 after 6 h and decreased activity was observed after 9 h of application. The OsRGLP2 promoter also contains cis-acting elements, which are responsive to drought, SA, NaCl, H2O2 and auxin. Analysis of this stress inducible promoter can be useful in studying its potential use in developing stress tolerance in plants.
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