Osmotic water permeability of capillaries from the isolated spiral ligament: new in-vitro techniques for the study of vascular permeability and diameter

Abstract

Perilymph is separated from blood by a barrier called the blood-labyrinth or blood-perilymph barrier in analogy to the blood-brain or blood-cerebrospinal fluid barrier. These barriers consist mainly of vascular endothelial cells. To characterize the blood-labyrinth barrier we developed in vitro techniques for the quantitative determination of the osmotic water permeability and for the determination of changes in the diameter of isolated inner ear capillaries. Both techniques rely on measurement of the velocity of marker red cells trapped in the lumen of capillaries. The velocity of marker red cells is a measure for the capillary permeability when a water flux across the capillary wall is induced by an osmotic gradient or a measure for a change in the capillary diameter. With these techniques the osmotic water permeability coefficient ( P f ) and the pH sensitivity of isolated capillaries from the spiral ligament of the inner ear was determined. P f at 23°C was (1.49 ± 0.17) 10 −3 cm/s at pH 7.4 and (1.61 ± 0.23) 10 −3 cm/s at pH 6.8 ( n = 12; mean ± SEM; n = number of tissues). P f at 37°C was (2.26 ± 0.23) 10 −3 cm/s at pH 7.4 and (2.35 ± 0.17) 10 −3 cm/s at pH 6.8 ( n = 13). No change in capillary diameter was observed when the pH of the interstitial fluid was lowered from pH 7.4 to 6.8. These data demonstrate that Pf and the capillary diameter of spiral ligament capillaries are pH independent and suggest that water crosses the blood-labyrinth barrier via an aqueous pathway. Further, these data suggest that the relatively low P f is another characteristic shared by the blood-labyrinth and the blood-brain barrier.