Osmotic Stress and Ion-Specific Effects on Xylem Abscisic Acid and the Relevance to Salinity Tolerance in Poplar

Abstract

We designed two experiments to investigate the osmotic stress and ion-specific effects on xylem abscisic acid (ABA) and the relevance to salinity tolerance in one-year-old seedlings of Populus euphratica Oliv. (a salt-resistant genotype) and one-year-old rooted cuttings of P. 'popularis 35-44' (P. popularis) (a salt-sensitive genotype). Net photosynthetic rates (Pn) and unit transpiration rates (TRN) of the two genotypes were significantly decreased upon osmotic shock caused by PEG 6000 (osmotic potential = -0.24 MPa) or iso-NaCl (50 mM). Shoot xylem ABA concentrations in both genotypes increased rapidly after the onset of PEG stress, resulting from a decreased water flow. NaCl-treated trees of P. euphratica maintained considerably greater concentrations of ABA than PEG-treated plants in a longer term, whereas salinized P. popularis exhibited a transient accumulation of ABA in the shoot. TRN was greatly enhanced in both genotypes when pressure (0.24 MPa) was applied to counteract the osmotic suction of 50 mM NaCl. Pressurizing of root systems diluted solutes in the root xylem, but the dilution effect was more pronounced in P. popularis. Root xylem ABA concentrations in P. euphratica steadily increased with salt stress although pressurization lowered its levels. In contrast, there were no observed changes in ABA response to salinity in pressured P. popularis. Therefore, we concluded that the salt-tolerant P. euphratica had a greater capacity to synthesize ABA under saline conditions, which may partially result from specific salt effects. In addition, P. euphratica exhibited a higher capacity for salt (Na+ and Cl-) transport control under salt stress, compared with P. popularis. The possible association between ABA and salt transport limitation, and the relevance to salinity tolerance were discussed.