Abstract
Osmotic properties of mitochondria isolated from Acanthamoeba sp. were studied. Included were volumes of amoeba mitochondria in a wide variety of suspending media, a comparison of actual mitochondrial volumes determined by centrifugal packing with volumes determined by the optical density method, and mitochondrial metabolism at different volumes. Amoeba mitochondria were found to have many properties similar to those from mammalian tissues. Particle volume changes were likened to biocolloid effects rather than to osmotic phenomena. The unreliability of the optical density method in determining mitochondrial volumes was demonstrated. The role of intracellular tonicity and the site of citrate and succinate oxidation were discussed.
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