Osmotic, ionic and haematological response of the Port Jackson shark Heterodontus portusjacksoni and the common stingaree Trygonoptera testacea upon exposure to diluted seawater

Abstract

The osmo-ionoregulatory responses of sub-adult Port Jackson sharks, Heterodontus portusjacksoni (Meyer, 1793), and the stingaree Trygonoptera testacea (Muller and Henle, 1841), were investigated following the transfer from full-strength seawater (100% SW) to either 75 or 50% SW for up to 168 h. The plasma osmotic pressure was similarly reduced in both species and remained slightly hyper-osmotic to the diluted seawater after 168 h. The increase in the body weight of H.␣portusjacksoni transferred to diluted seawater was double that of T.testacea, with the greater haemodilution reflected by a short-term reduction in haematocrit and in haemoglobin concentration. The haemodilution exhibited by H. portusjacksoni also resulted in a greater reduction in plasma [Na], [Cl], [urea] and [trimethylamine oxide] compared with T. testacea. The ability of T. testacea to minimize body-weight gain in diluted seawater was most probably due to increased net water efflux. The persistent elevation in the body weight of both species, despite the apparent recovery of plasma-water volume, was indicative of elevated tissue water. Chronic exposure of H. portusjacksoni to 50% SW altered erythrocyte ion concentrations and plasma/erythrocyte ion ratios, which could have serious implications for cellular homeostasis, respiratory gas transport and acid-base status. The greater control of water and ionic status exhibited by T.testacea, compared with that of H.portusjacksoni, allows the stingarees to penetrate into the more dilute, upper estuarine waters.