Abstract
Experiments were conducted in which normal human red blood cells (RBC) were subjected to osmotic hemolysis (hypotonic NaCl and urea) in the presence of different concentrations (0–20 mM) of three surface active substances (Pluronic® F 38, F 68, F 108). All three substances exhibited an antihemolytic effect which increased with increasing concentrations and was most pronounced in the case of F 108. Measurements of intracellular osmolarity, hemoglobin concentration, RBC count, and hematocrit showed that in the presence of 2 mM/F 108 the cells exhibit an increased critical hemolytic volume. Osmotic equilibrium with the suspending medium is achieved by water influx through the membrane which is not inhibited by Pluronic. It is conceivable that in the presence of Pluronic the RBC membrane becomes more resistant against tangential stresses.
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