Abstract
Abstract 1. 1. When isolated spinach chloroplasts were incubated in the cold in various dilutions of NaCl, sucrose or spinach juice media, the chloroplast volume was found to vary inversely with osmotic pressure of the medium in accordance with the Boyle-Van 't Hoff relation. 2. 2. These volume changes were reversible. 3. 3. Electrolyte analyses of pellets and supernatants indicated that Na + , Cl − , and K + readily penetrated the chloroplast membrane. These results raise the question of whether the observed swelling and shrinking of isolated chloroplasts can be explained in terms of simple semipermeable-membrane equilibria.
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