Abstract
1. 1. Larval and adult A. tigrinum maintain NaCl balance in artificial pond water even when starved. 2. 2. Sodium is lost in the urine and across the body surface at a mean rate of 1·3 μg-equiv/10 g per hr. Urinary loss represents 60–90 per cent of the total efflux in larvae and 55 per cent of the total in adults. 3. 3. sodium loss is balanced by uptake across the body surface against a large gradient of electrochemical potential. Chloride is also actively transported inward. 4. 4. Osmotic influx of water occurs at the rate of 26 per cent of body weight per day in larvae, 32 per cent of body weight per day in adults. The osmotic steady state is preserved by excretion of an equivalent volume of dilute urine. 5. 5. Sodium and chloride concentrations were lower in adult than in larval urine.
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