Abstract
Osmotic adjustment is a persisting controversy in studies on the effect of salt and water stress in cowpea crops. Our hypothesis is that the osmotic potential determination method interferes with the osmotic adjustment calculation. The objective of this study was to consider the osmotic adjustment comparing results obtained by pressure-volume (P–V) curves and osmometry. The experiment was conducted in a randomized block design, with six salt water concentrations 0, 20, 40, 60, 80, and 100 mmol L−1 of NaCl in a Fluvisol. The osmotic adjustment found through osmometry were lower than those found through P–V curves. The apoplastic water fraction of the cowpea had a dilution effect, denoting overestimation of the osmotic potential by the methodology based on osmometry. This may be the source of the different interpretations of osmotic adjustment in cowpea plants. Thus, osmotic adjustment should be calculated preferably using the osmotic potential determined by method of pressure-volume curves.
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