Osmosensitive hypothalamic neurons and their responses to cardiovascular receptor activation

Abstract

Neurons in the rostral hypothalamic areas were examined with physiologically hypertonic (+30 mOsm/kg, by NaCl or mannitol) and hypotonic (−30 mOsm/kg) artificial cerebrospinal fluids (ACSFs) applied by pressure through a multibarrel micropipette in urethane-anesthetized rats. Of 304 neurons tested, 39 were excited by the hyportonic ACSFs and/or inhibited by the hypotonic ACSF, and 35 were inhibited by the hyportonic ACSFs and/or excited by the hypotonic ACSF. The former cells were designated hypertonic-sensitive and the latter hypotonic-sensitive. Both types of osmosensitive neurons were diffusely scattered in the examined areas, but neurons in the lateral preoptic area and the bed nucleus of the stria terminalis responded more frequently (30–40%) to the osmotic stimuli. Osmosensitive and insensitive neurons were recorded during activation of the baro- and volume receptors of the cardiovascular system. Of seven neurons that were excited during temporal hypotension induced by intravenous administration of nitroprus-side, five were hyportonic-sensitive and two were osmotically insensitive. Hypertonic-sensitive neurons may be activated during dehydration, which increases the osmotic pressure and decreases the volume of body fluids. Of six neurons that were excited during temporal hypertension induced by intravenous administration of phenylephrine, four were hypotonic-sensitive and two were osmotically insensitive. Hypotonic-sensitive neurons may be activated during rehydration or overhydration. Osmosensitive neurons probably integrate cardiovascular and osmotic information that is important for the central regulation of body fluids.