Osmoregulation in the mudskipper,Boleophthalmus boddaerti I. Responses of branchial cation activated and anion stimulated adenosine triphosphatases to changes in salinity

Abstract

The mudskipperB. boddaerti, was able to survive in waters of intermediate salinities (4-27‰). Fish submerged in dechlorinated tap water suffered 60% mortality by the fifth day while 60% of those in 100% sea-water (sw) died after the third day of exposure. After being submerged in 50% or 80% sw for 7 days, the plasma osmolality, plasma Na(+) and Cl(-) concentrations and the branchial Na(+) and K(+) activated adenosine triphosphatase (Na(+),K(+)-ATPase) activity were significantly higher than those of fish submerged in 10% sw for the same period. However, the activities of the branchial HCO3 (-) and Cl(-) stimulated adenosine triphosphatase (HCO3 (-),Cl(-)-ATPase) and carbonic anhydrase of the latter fish were significantly greater than those of the former. Such correlation suggests that Na(+),K(+)-ATPase is important for hyperosmotic adaptation in this fish while HCO3 (-)-Cl(-)-ATPase and carbonic anhydrase may be involved in hypoosmotic survival.