Osmoregulation and cell volume regulation in the preimplantation embryo

Abstract

The early preimplantation mammalian embryo possesses mechanisms that regulate intracellular osmolarity and cell volume. While transport of osmotically active inorganic ions might play a role in this process in embryos, the major mechanisms that have been identified and studied are those that employ organic osmolytes. Organic osmolytes provide a substantial portion of intracellular osmotic support in embryos and are required for their development under in vivo conditions. The main osmolytes that have been identified in cleavage stage embryos are accumulated via two transport systems of the neurotransmitter transporter family active in early preimplantation embryos--the glycine transport system (GLY) and the beta-amino acid transport system (system beta). While system beta has been established to have a similar role in many other cells, this is a novel function for the GLY transport system. The intracellular concentration of organic osmolytes such as glycine in early preimplantation embryos is regulated by tonicity, allowing the embryo to regulate its volume against shrinkage and to control its internal osmolarity. In addition, the cells of the embryo can regulate against an increase in volume via controlled release of osmolytes from the cytoplasm. This is mediated by a swelling-activated anion channel that is also highly permeable to a range of organic osmolytes, and which closely resembles similar channels found in many other cell types (VSOAC channels). Together, these mechanisms appear to regulate cell volume in the egg through the early cleavage stages of embryogenesis, after which there are indications that the mechanisms of osmoregulation change.