Abstract
Meiosis plays an essential role in the production of gametes and genetic diversity of posterities. The normal double-strand break (DSB) repair is vital to homologous recombination (HR) and occurrence of DNA fragment exchange, but the underlying molecular mechanism remain elusive. Here, we characterized a completely sterile Osmfs1 (male and female sterility 1) mutant which has its pollen and embryo sacs both aborted at the reproductive stage due to severe chromosome defection. Map-based cloning revealed that the OsMFS1 encodes a meiotic coiled-coil protein, and it is responsible for DSB repairing that acts as an important cofactor to stimulate the single strand invasion. Expression pattern analyses showed the OsMFS1 was preferentially expressed in meiosis stage. Subcellular localization analysis of OsMFS1 revealed its association with the nucleus exclusively. In addition, a yeast two-hybrid (Y2H) and pull-down assay showed that OsMFS1 could physically interact with OsHOP2 protein to form a stable complex to ensure faithful homologous recombination. Taken together, our results indicated that OsMFS1 is indispensable to the normal development of anther and embryo sacs in rice.
Highlights
Meiosis is indispensable for sexual reproduction in eukaryotes, and is a specialized cellular division process
We showed the interaction between OsMFS1 and OsHOP2 by Y2H and pull-down assay in rice, which were observed in yeasts, mammals, and Arabidopsis thaliana (Hideo and Shirleen, 2002; Petukhova et al, 2005; Claudia et al, 2006)
The Oshop2 mutants shared the same chromosomal defects with Osmfs1, suggesting that the OsMFS1 and the OsHOP2 acted in the same pathway forming heterodimeric complex to stimulate double-strand break (DSB) repair
Summary
Meiosis is indispensable for sexual reproduction in eukaryotes, and is a specialized cellular division process. In this process, the sexually reproducing cells undergo a round of DNA replication and two successive rounds of nuclear division, and form genetically recombined haploid generative cells (Dawe, 1998; Kleckner, 2006). During the second division (meiosis II), the sister chromatids separate to form four genetically different haploid cells. Compared with mitosis, these two chromosome segregations of meiosis possess a great amount of innovations (Ma, 2006; Mercier et al, 2015; Gray and Cohen, 2016).
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